Review



addgene plasmid  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Addgene inc addgene plasmid
    Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/addgene+dsred+rab11/DsRed-rab11+WT+(Plasmid+%2312679)/pmc12796769-67-7-7
    Average 93 stars, based on 57 article reviews
    addgene plasmid - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Expressing:

    Article Title: CROP2, a Retriever-PROPPIN Complex Mediating Protein Export from Endosomes to the Plasma Membrane
    Article Snippet: Phosphatase inhibitor cocktail final concentrations: 10 mM NaF (S-7920, Sigma); 1mM Na Orthovanadate (S6508, Sigma); 2 mM β-glycerophosphate (A2253, AppliChem) all dissolved in water. .. Vectors expressing tagged RAB proteins were purchased from Addgene: DsRed- RAB11 (12679; deposited by R. Pagano); mCherry-RAB7 (61804; deposited by G. Voeltz); mCherry-RAB5 (49201; deposited by G. Voeltz). .. The following vectors were kindly provided by colleagues: EGFP-WIPI2 (pAR31CD vector) (Tassula Proikas- Cezanne, Tübingen, Germany).



    Similar Products

    addgene plasmid  (Addgene inc)
    93
    Addgene inc addgene plasmid
    Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/addgene+dsred+rab11/DsRed-rab11+WT+(Plasmid+%2312679)/pmc12796769-67-7-7
    Average 93 stars, based on 1 article reviews
    addgene plasmid - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    addgene dsred rab11  (Addgene inc)
    93
    Addgene inc addgene dsred rab11
    Role of the WIPI2 FSSS motif for recruiting WIPI2 and Retriever to <t>Rab11</t> endosomes . WIPI2-knockdown HK2 cells were transfected with plasmids expressing the indicated siRNA- resistant EGFP WIPI2 variants and mCherry-RAB5, mCherry-RAB7, or DsRed-Rab11 were fixed, permeabilized, immuno-stained for VPS35L and analyzed by confocal microscopy. Examples of the quantified images are presented in Supplementary Figures S5 to S7. Colocalization with EGFP WIPI2 and VPS35L was assessed for: A. RAB11 B. RAB5 C. RAB7. Colocalization was quantified using Manders’ colocalization coefficient M2, calculated in ImageJ. M2 refers to the fraction of VPS35L colocalising with the different Rab-proteins. For the triple colocalizations WIPI2/VPS35L/RAB M2 indicates the fraction of green pixels (WIPI2) overlapping with the pixels positive for the VPS35L/RAB colocalization. Colocalization was quantified from three independent experiments in a total of 120 cells. P values were calculated applying Welch’s t-test with unequal variances. The analysis was performed with 99% confidence. ***P < 0.0001.
    Addgene Dsred Rab11, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/addgene+dsred+rab11/DsRed-rab11+WT+(Plasmid+%2312679)/bio_rxiv__2025__10__08__681146-154-8-8
    Average 93 stars, based on 1 article reviews
    addgene dsred rab11 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    addgene 12679 pegfp n1 clontech  (Addgene inc)
    93
    Addgene inc addgene 12679 pegfp n1 clontech
    Role of the WIPI2 FSSS motif for recruiting WIPI2 and Retriever to <t>Rab11</t> endosomes . WIPI2-knockdown HK2 cells were transfected with plasmids expressing the indicated siRNA- resistant EGFP WIPI2 variants and mCherry-RAB5, mCherry-RAB7, or DsRed-Rab11 were fixed, permeabilized, immuno-stained for VPS35L and analyzed by confocal microscopy. Examples of the quantified images are presented in Supplementary Figures S5 to S7. Colocalization with EGFP WIPI2 and VPS35L was assessed for: A. RAB11 B. RAB5 C. RAB7. Colocalization was quantified using Manders’ colocalization coefficient M2, calculated in ImageJ. M2 refers to the fraction of VPS35L colocalising with the different Rab-proteins. For the triple colocalizations WIPI2/VPS35L/RAB M2 indicates the fraction of green pixels (WIPI2) overlapping with the pixels positive for the VPS35L/RAB colocalization. Colocalization was quantified from three independent experiments in a total of 120 cells. P values were calculated applying Welch’s t-test with unequal variances. The analysis was performed with 99% confidence. ***P < 0.0001.
    Addgene 12679 Pegfp N1 Clontech, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/addgene+dsred+rab11/DsRed-rab11+WT+(Plasmid+%2312679)/pm31775031-166-107-107
    Average 93 stars, based on 1 article reviews
    addgene 12679 pegfp n1 clontech - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    c1 rab11 addgene  (Addgene inc)
    93
    Addgene inc c1 rab11 addgene
    Role of the WIPI2 FSSS motif for recruiting WIPI2 and Retriever to <t>Rab11</t> endosomes . WIPI2-knockdown HK2 cells were transfected with plasmids expressing the indicated siRNA- resistant EGFP WIPI2 variants and mCherry-RAB5, mCherry-RAB7, or DsRed-Rab11 were fixed, permeabilized, immuno-stained for VPS35L and analyzed by confocal microscopy. Examples of the quantified images are presented in Supplementary Figures S5 to S7. Colocalization with EGFP WIPI2 and VPS35L was assessed for: A. RAB11 B. RAB5 C. RAB7. Colocalization was quantified using Manders’ colocalization coefficient M2, calculated in ImageJ. M2 refers to the fraction of VPS35L colocalising with the different Rab-proteins. For the triple colocalizations WIPI2/VPS35L/RAB M2 indicates the fraction of green pixels (WIPI2) overlapping with the pixels positive for the VPS35L/RAB colocalization. Colocalization was quantified from three independent experiments in a total of 120 cells. P values were calculated applying Welch’s t-test with unequal variances. The analysis was performed with 99% confidence. ***P < 0.0001.
    C1 Rab11 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/addgene+dsred+rab11/DsRed-rab11+WT+(Plasmid+%2312679)/pm31054872-270-81-82
    Average 93 stars, based on 1 article reviews
    c1 rab11 addgene - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    pmcherry rab4a 7 addgene  (Addgene inc)
    93
    Addgene inc pmcherry rab4a 7 addgene
    Role of the WIPI2 FSSS motif for recruiting WIPI2 and Retriever to <t>Rab11</t> endosomes . WIPI2-knockdown HK2 cells were transfected with plasmids expressing the indicated siRNA- resistant EGFP WIPI2 variants and mCherry-RAB5, mCherry-RAB7, or DsRed-Rab11 were fixed, permeabilized, immuno-stained for VPS35L and analyzed by confocal microscopy. Examples of the quantified images are presented in Supplementary Figures S5 to S7. Colocalization with EGFP WIPI2 and VPS35L was assessed for: A. RAB11 B. RAB5 C. RAB7. Colocalization was quantified using Manders’ colocalization coefficient M2, calculated in ImageJ. M2 refers to the fraction of VPS35L colocalising with the different Rab-proteins. For the triple colocalizations WIPI2/VPS35L/RAB M2 indicates the fraction of green pixels (WIPI2) overlapping with the pixels positive for the VPS35L/RAB colocalization. Colocalization was quantified from three independent experiments in a total of 120 cells. P values were calculated applying Welch’s t-test with unequal variances. The analysis was performed with 99% confidence. ***P < 0.0001.
    Pmcherry Rab4a 7 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/addgene+dsred+rab11/DsRed-rab11+WT+(Plasmid+%2312679)/pm31054872-270-89-90
    Average 93 stars, based on 1 article reviews
    pmcherry rab4a 7 addgene - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    kanr addgene 12679 pegfp c1 c  (Addgene inc)
    93
    Addgene inc kanr addgene 12679 pegfp c1 c
    Role of the WIPI2 FSSS motif for recruiting WIPI2 and Retriever to <t>Rab11</t> endosomes . WIPI2-knockdown HK2 cells were transfected with plasmids expressing the indicated siRNA- resistant EGFP WIPI2 variants and mCherry-RAB5, mCherry-RAB7, or DsRed-Rab11 were fixed, permeabilized, immuno-stained for VPS35L and analyzed by confocal microscopy. Examples of the quantified images are presented in Supplementary Figures S5 to S7. Colocalization with EGFP WIPI2 and VPS35L was assessed for: A. RAB11 B. RAB5 C. RAB7. Colocalization was quantified using Manders’ colocalization coefficient M2, calculated in ImageJ. M2 refers to the fraction of VPS35L colocalising with the different Rab-proteins. For the triple colocalizations WIPI2/VPS35L/RAB M2 indicates the fraction of green pixels (WIPI2) overlapping with the pixels positive for the VPS35L/RAB colocalization. Colocalization was quantified from three independent experiments in a total of 120 cells. P values were calculated applying Welch’s t-test with unequal variances. The analysis was performed with 99% confidence. ***P < 0.0001.
    Kanr Addgene 12679 Pegfp C1 C, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/addgene+dsred+rab11/DsRed-rab11+WT+(Plasmid+%2312679)/pmc06021133__NIHMS976622___supplement___1-5-23-24
    Average 93 stars, based on 1 article reviews
    kanr addgene 12679 pegfp c1 c - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Role of the WIPI2 FSSS motif for recruiting WIPI2 and Retriever to Rab11 endosomes . WIPI2-knockdown HK2 cells were transfected with plasmids expressing the indicated siRNA- resistant EGFP WIPI2 variants and mCherry-RAB5, mCherry-RAB7, or DsRed-Rab11 were fixed, permeabilized, immuno-stained for VPS35L and analyzed by confocal microscopy. Examples of the quantified images are presented in Supplementary Figures S5 to S7. Colocalization with EGFP WIPI2 and VPS35L was assessed for: A. RAB11 B. RAB5 C. RAB7. Colocalization was quantified using Manders’ colocalization coefficient M2, calculated in ImageJ. M2 refers to the fraction of VPS35L colocalising with the different Rab-proteins. For the triple colocalizations WIPI2/VPS35L/RAB M2 indicates the fraction of green pixels (WIPI2) overlapping with the pixels positive for the VPS35L/RAB colocalization. Colocalization was quantified from three independent experiments in a total of 120 cells. P values were calculated applying Welch’s t-test with unequal variances. The analysis was performed with 99% confidence. ***P < 0.0001.

    Journal: bioRxiv

    Article Title: CROP2, a Retriever-PROPPIN Complex Mediating Protein Export from Endosomes to the Plasma Membrane

    doi: 10.1101/2025.10.08.681146

    Figure Lengend Snippet: Role of the WIPI2 FSSS motif for recruiting WIPI2 and Retriever to Rab11 endosomes . WIPI2-knockdown HK2 cells were transfected with plasmids expressing the indicated siRNA- resistant EGFP WIPI2 variants and mCherry-RAB5, mCherry-RAB7, or DsRed-Rab11 were fixed, permeabilized, immuno-stained for VPS35L and analyzed by confocal microscopy. Examples of the quantified images are presented in Supplementary Figures S5 to S7. Colocalization with EGFP WIPI2 and VPS35L was assessed for: A. RAB11 B. RAB5 C. RAB7. Colocalization was quantified using Manders’ colocalization coefficient M2, calculated in ImageJ. M2 refers to the fraction of VPS35L colocalising with the different Rab-proteins. For the triple colocalizations WIPI2/VPS35L/RAB M2 indicates the fraction of green pixels (WIPI2) overlapping with the pixels positive for the VPS35L/RAB colocalization. Colocalization was quantified from three independent experiments in a total of 120 cells. P values were calculated applying Welch’s t-test with unequal variances. The analysis was performed with 99% confidence. ***P < 0.0001.

    Article Snippet: Vectors expressing tagged RAB proteins were purchased from Addgene: DsRed- RAB11 (12679; deposited by R. Pagano); mCherry-RAB7 (61804; deposited by G. Voeltz); mCherry-RAB5 (49201; deposited by G. Voeltz).

    Techniques: Knockdown, Transfection, Expressing, Staining, Confocal Microscopy